Canyon mouse (Peromyscus crinitus)
Vocalizations are an important form of communication in canyon mice. When injured, adults make brief squeaking sounds, and when defending the nest, they often make "chit" sounds. When excited, canyon mice thump the ground with their hind feet, and when aggressive, they chatter their teeth! Read more about canyon mice on Animal Diversity Web.
Photo by Matthew MacManes
Chromosome-length genome assembly
Download the pecr10X_v2_HiC.fasta.gz file containing the chromosome-length (2n=48) assembly of the canyon mouse genome. All modifications with respect to the draft (see below) are annotated in the pecr10X_v2_HiC.assembly file. Some basic stats associated with the new reference, pecr10X_v2_HiC, are listed below. The full data release can be explored here.
Contig length (bp) | Number of contigs | Contig N50 (bp) | Longest contig (bp) |
|---|---|---|---|
2,490,976,159 | 78,433 | 137,026 | 1,011,342 |
Scaffold length (bp) | Number of scaffolds | Scaffold N50 (bp) | Longest scaffold (bp) |
|---|---|---|---|
2,501,959,427 | 43,773 | 97,468,232 | 173,176,752 |
Draft
The chromosome-length genome assembly is based on the draft assembly pecr10X_v2, credited below.
The chromosome-length genome assembly was created in collaboration with the MacManes lab, University of New Hampshire. The draft genome assembly was generated using 10X data by Matthew MacManes, Anna Tigano and Jocelyn Colella, University of New Hampshire.
Method
3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018). See Methods for more information.
Hi-C sample
The fibroblast sample for in situ Hi-C preparation was donated by a female individual, and obtained from MD Anderson Frozen Archive.
Hi-C Contact maps
Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). (Please note that the interactive figures are scaled 1:2.) To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018).
References
If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.
The chromosome-length genome assembly was created in collaboration with the MacManes lab, University of New Hampshire. The draft genome assembly was generated using 10X data by Matthew MacManes, Anna Tigano and Jocelyn Colella, University of New Hampshire.
Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.
Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.
Disclaimer
This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at thednazoo@gmail.com or leave a comment on the Forum.