Clouded leopard (Neofelis nebulosa)
Clouded leopards have the longest, in relation to body size, of any cat's tail! The tail gives the clouded leopard great balance when strolling along tree branches. Read more about clouded leopards on Smithsonian's National Zoo website.
Clouded leopard at the national zoo, by Brian Gratwicke [CC BY 2.0]
Chromosome-length genome assembly
Download the Neofelis_nebulosa_HiC.fasta.gz file containing the chromosome-length (2n=38) assembly of the clouded leopard genome. All modifications with respect to the draft (see below) are annotated in the Neofelis_nebulosa_HiC.assembly file. Some basic stats associated with the new reference, Neofelis_nebulosa_HiC, are listed below. The full data release can be explored here.
Contig length (bp) | Number of contigs | Contig N50 (bp) | Longest contig (bp) |
|---|---|---|---|
2,408,244,115 | 84,944 | 76,417 | 651,517 |
Scaffold length (bp) | Number of scaffolds | Scaffold N50 (bp) | Longest scaffold (bp) |
|---|---|---|---|
2,416,186,965 | 12,793 | 147,111,411 | 239,539,317 |
Draft
The chromosome-length genome assembly is based on the draft assembly Neofelis_nebulosa, credited below.
The draft genome assembly was generated by Paul Frandsen (Brigham Young University) and Madeline Bursell (Brigham Young University) in collaboration with Warren Johnson (Smithsonian Conservation Biology Institute), Klaus-Peter Koepfli (Smithsonian Conservation Biology Institute) and Rebecca Dikow (Smithsonian Institution Data Science Lab). The draft assembly was generated using MaSuRCA.
Method
3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018). See Methods for more information.
Hi-C sample
The blood sample for in situ Hi-C preparation was donated by a female individual named Rhu, and provided to us by Houston Zoo.
Hi-C Contact maps
Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). (Please note that the interactive figures are scaled 1:2.) To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018).
References
If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.
The draft genome assembly was generated by Paul Frandsen (Brigham Young University) and Madeline Bursell (Brigham Young University) in collaboration with Warren Johnson (Smithsonian Conservation Biology Institute), Klaus-Peter Koepfli (Smithsonian Conservation Biology Institute) and Rebecca Dikow (Smithsonian Institution Data Science Lab). The draft assembly was generated using MaSuRCA.
Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.
Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.
Disclaimer
This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at thednazoo@gmail.com or leave a comment on the Forum.