Little brown bat (Myotis lucifugus)
Little brown bats feed on a great variety of small, flying insects. They locate these insects using echolocation. An average bat will consume as many as 1000 insects, while a pregnant female will eat up to her whole body weight per sitting! Read more about little brown bats on Hinterland Who's Who website.
By U. S. Fish and Wildlife Service - Northeast Region - https://www.flickr.com/photos/usfwsnortheast/5571233941/, Public Domain, https://commons.wikimedia.org/w/index.php?curid=51936277
Chromosome-length genome assembly
Download the Myoluc2.0_HiC.fasta.gz file containing the chromosome-length (2n=44) assembly of the little brown bat genome. All modifications with respect to the draft (see below) are annotated in the Myoluc2.0_HiC.assembly file. Some basic stats associated with the new reference, Myoluc2.0_HiC, are listed below. The full data release can be explored here.
Contig length (bp)
Number of contigs
Contig N50 (bp)
Longest contig (bp)
Scaffold length (bp)
Number of scaffolds
Scaffold N50 (bp)
Longest scaffold (bp)
The chromosome-length genome assembly is based on the draft assembly Myoluc2.0 (GCF_000147115.1), credited below.
3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018). See Methods for more information.
The primary sample for in situ Hi-C preparation were primary dermal fibroblasts donated by a female individual, and obtained from Vincent Lynch (University of Buffalo), Juan Vazquez (University of Chicago) and Richard Miller (University of Michigan).
Hi-C Contact maps
Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018).
If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.
Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.
Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.
This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at email@example.com or leave a comment on the Forum.