Helmeted honeyeater (Lichenostomus melanops cassidix)
The helmeted honeyeater is an endangered subspecies of bird. It is the Australia state of Victoria's official bird emblem! Read more about helmeted honeyeater in KidzSearch encyclopedia for kids.
Helmeted Honeyeater (Lichenostomus melanops cassidix). Photo by Bruce Lyon.
Chromosome-length genome assembly
Download the HeHo_1.0_HiC.fasta.gz file containing the chromosome-length assembly of the helmeted honeyeater genome. All modifications with respect to the draft (see below) are annotated in the HeHo_1.0_HiC.assembly file. Some basic stats associated with the new reference, HeHo_1.0_HiC, are listed below. The full data release can be explored here.
Contig length (bp) | Number of contigs | Contig N50 (bp) | Longest contig (bp) |
|---|---|---|---|
1,102,300,766 | 2,239 | 6,736,108 | 42,472,965 |
Scaffold length (bp) | Number of scaffolds | Scaffold N50 (bp) | Longest scaffold (bp) |
|---|---|---|---|
1,102,960,466 | 906 | 63,800,663 | 152,677,470 |
Draft
The chromosome-length genome assembly is based on the draft assembly HeHo_1.0 (GCA_008360975.1), credited below.
Gan, H.M., Pavlova, A., Ruiz, D.R. and Sunnucks, P. "Genome sequencing of the critically endangered Helmeted Honeyeater." Unpublished. https://www.ncbi.nlm.nih.gov/nuccore/VLJF00000000.1/.
Method
3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018). See Methods for more information.
Hi-C sample
The liver sample for in situ Hi-C preparation was donated by a female individual, and obtained from Yellingbo Nature Conservation Reserve, via Leanne Wicker (Zoos Victoria) and Alexandra Pavlova (Monash University, Melbourne).
Hi-C Contact maps
Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018).
References
If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.
Gan, H.M., Pavlova, A., Ruiz, D.R. and Sunnucks, P. "Genome sequencing of the critically endangered Helmeted Honeyeater." Unpublished. https://www.ncbi.nlm.nih.gov/nuccore/VLJF00000000.1/.
Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.
Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.
Disclaimer
This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at thednazoo@gmail.com or leave a comment on the Forum.