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Cucurbita maxima cultivated pumpkin (Cucurbita maxima)

Cucurbita maxima, one of at least four species of cultivated squash, is one of the most diverse domesticated species! Read more about Cucurbita maxima cultivated pumpkins on Wikipedia.

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Cucurbita maxima (Brodee galeuse d'Eysines) Cl J Weber01, photo by INRA Jean Weber [CC 2.0] via flickr.com

Chromosome-length genome assembly

Download the Cmax_1.0_HiC.fasta.gz file containing the chromosome-length (n=20) assembly of the Cucurbita maxima cultivated pumpkin genome. All modifications with respect to the draft (see below) are annotated in the Cmax_1.0_HiC.assembly file. Some basic stats associated with the new reference, Cmax_1.0_HiC, are listed below. The full data release can be explored here.

Contig length (bp)
Number of contigs
Contig N50 (bp)
Longest contig (bp)
265,447,813
25,556
40,549
400,020
Scaffold length (bp)
Number of scaffolds
Scaffold N50 (bp)
Longest scaffold (bp)
271,635,397
7,885
12,071,977
20,800,366
Draft

The chromosome-length genome assembly is based on the draft assembly Cmax_1.0 (GCA_002738345.1), credited below.

Sun, H., Wu, S., Zhang, G., Jiao, C., Guo, S., Ren, Y., Zhang, J., Zhang, H., Gong, G., Jia, Z., Zhang, F., Tian, J., Lucas, W.J., Doyle, J.J., Li, H., Fei, Z., Xu, Y., 2017. Karyotype Stability and Unbiased Fractionation in the Paleo-Allotetraploid Cucurbita Genomes. Mol. Plant 10, 1293-1306. https://doi.org/10.1016/j.molp.2017.09.003.

Method

3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools  (Dudchenko et al., bioRxiv, 2018). See Methods for more information.

Hi-C sample

The seeds sample for in situ Hi-C preparation was from Galeux D' Eysines heirloom, obtained from Pinetree Garden Seeds.

Hi-C Contact maps

Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools  (Dudchenko et al., bioRxiv, 2018).

References

If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.

Sun, H., Wu, S., Zhang, G., Jiao, C., Guo, S., Ren, Y., Zhang, J., Zhang, H., Gong, G., Jia, Z., Zhang, F., Tian, J., Lucas, W.J., Doyle, J.J., Li, H., Fei, Z., Xu, Y., 2017. Karyotype Stability and Unbiased Fractionation in the Paleo-Allotetraploid Cucurbita Genomes. Mol. Plant 10, 1293-1306. https://doi.org/10.1016/j.molp.2017.09.003.

Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.

Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.

Disclaimer

This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at thednazoo@gmail.com or leave a comment on the Forum.

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