Golden eagle (Aquila chrysaetos)
Golden eagles are found in North America, Europe, Asia and North Africa: it is the most widely distributed species of eagle! Read more about golden eagles on Audubon website.
Photo by Manfred Richter [Pixabay License], from pixabay.com
Chromosome-length genome assembly
Download the Aquila_chrysaetos-1.0.2_HiC.fasta.gz file containing the chromosome-length assembly of the golden eagle genome. All modifications with respect to the draft (see below) are annotated in the Aquila_chrysaetos-1.0.2_HiC.assembly file. Some basic stats associated with the new reference, Aquila_chrysaetos-1.0.2_HiC, are listed below. The full data release can be explored here.
Contig length (bp) | Number of contigs | Contig N50 (bp) | Longest contig (bp) |
|---|---|---|---|
1,180,017,133 | 18,956 | 146,717 | 928,912 |
Scaffold length (bp) | Number of scaffolds | Scaffold N50 (bp) | Longest scaffold (bp) |
|---|---|---|---|
1,192,902,703 | 797 | 46,579,469 | 86,574,484 |
Draft
The chromosome-length genome assembly is based on the draft assembly Aquila_chrysaetos-1.0.2 (GCA_000766835.1), credited below.
Bussche, Ronald A. Van Den, Megan E. Judkins, Michael J. Montague, and Wesley C. Warren. 2017. "A Resource of Genome-Wide Single Nucleotide Polymorphisms (Snps) for the Conservation and Management of Golden Eagles." Journal of Raptor Research 51 (3): 368-78. https://doi.org/10.3356/JRR-16-47.1.
Method
3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018). See Methods for more information.
Hi-C sample
The blood sample for in situ Hi-C preparation was donated by a female individual named Bonnie, and provided to us by Texas State Aquarium.
Hi-C Contact maps
Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018).
References
If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.
Bussche, Ronald A. Van Den, Megan E. Judkins, Michael J. Montague, and Wesley C. Warren. 2017. "A Resource of Genome-Wide Single Nucleotide Polymorphisms (Snps) for the Conservation and Management of Golden Eagles." Journal of Raptor Research 51 (3): 368-78. https://doi.org/10.3356/JRR-16-47.1.
Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.
Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.
Disclaimer
This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at thednazoo@gmail.com or leave a comment on the Forum.