Green anole (Anolis carolinensis)

Although they are closely related to iguanas, the green anole has the ability to change color like the chameleon. Read more about green anoles on the Wikipedia page.

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Green anole by Bonnie Kircher

Chromosome-length genome assembly

Download the AnoCar2.0_HiC.fasta.gz file containing the chromosome-length (2n=36) assembly of the green anole genome. All modifications with respect to the draft (see below) are annotated in the AnoCar2.0_HiC.assembly file. Some basic stats associated with the new reference, AnoCar2.0_HiC, are listed below. The full data release can be explored here.

Contig length (bp)
Number of contigs
Contig N50 (bp)
Longest contig (bp)
1,701,353,770
45,943
78,967
582,046
Scaffold length (bp)
Number of scaffolds
Scaffold N50 (bp)
Longest scaffold (bp)
1,792,895,096
7,904
279,943,727
360,608,016
Draft

The chromosome-length genome assembly is based on the draft assembly AnoCar2.0 (GCA_000090745.2), credited below.

Alföldi, J., Di Palma, F., Grabherr, M., Williams, C., Kong, L., Mauceli, E., Russell, P., Lowe, C.B., Glor, R.E., Jaffe, J.D., Ray, D. A., Boissinot, S., Shedlock, A.M., Botka, C., Castoe, T.A., Colbourne, J.K., Fujita, M.K., Moreno, R.G., ten Hallers, B.F., Haussler, D., … Lindblad-Toh, K., 2011. The genome of the green anole lizard and a comparative analysis with birds and mammals. Nature, 477(7366), 587-591. https://doi.org/10.1038/nature10390.

Method

3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools  (Dudchenko et al., bioRxiv, 2018). See Methods for more information.

Hi-C sample

The primary fibroblasts sample for in situ Hi-C preparation was donated by a female individual, and obtained from T.C. Hsu Cryo-Zoo at the University of Texas MD Anderson Cancer Center.

Hi-C Contact maps

Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools  (Dudchenko et al., bioRxiv, 2018).

References

If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.

Alföldi, J., Di Palma, F., Grabherr, M., Williams, C., Kong, L., Mauceli, E., Russell, P., Lowe, C.B., Glor, R.E., Jaffe, J.D., Ray, D. A., Boissinot, S., Shedlock, A.M., Botka, C., Castoe, T.A., Colbourne, J.K., Fujita, M.K., Moreno, R.G., ten Hallers, B.F., Haussler, D., … Lindblad-Toh, K., 2011. The genome of the green anole lizard and a comparative analysis with birds and mammals. Nature, 477(7366), 587-591. https://doi.org/10.1038/nature10390.

Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.

Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.

Disclaimer

This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at thednazoo@gmail.com or leave a comment on the Forum.