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Writer's pictureOlga Dudchenko

The Northern elephant seal Mirounga angustirostris nearly went extinct in the late 19th century. While the species made a dramatic recovery, with 200,000+ individuals alive today, they are all descendants of fewer than 100 individuals that found refuge on the Guadalupe Island, Mexico [1, 2].


Today, we are sharing a chromosome-length genome assembly for the Northern elephant seal (contig N50: 76 kb; scaffold N50: 140 Mb) generated entirely from short Illumina reads (PE150, see Methods for more details on the workflow). The samples for library construction have been collected at Año Nuevo State Park in California by Jane Khudyakov (University of the Pacific) and Daniel Crocker (Sonoma State University). We thank Ben Neely at NIST for coordinating the effort to collect these valuable samples!


Prior to the recent genome releases here at DNA Zoo, the only de novo chromosome-length assembly in the dog-like family (the suborder Caniformia) was the dog itself (Canis lupus familiaris), which diverged from seal ~50 million years ago. Below, you can see that the two species have very different karyotypes.


At DNA Zoo, we have now released eleven other Caniformia genomes: red panda (Ailurus fulgens), dingo (Canis lupus dingo), sea otter (Enhydra lutris), African wild dog (Lycaon pictus), domestic ferret (Mustela putorius furo), walrus (Odobenus rosmarus), common raccoon (Procyon lotor), American black bear (Ursus americanus), grizzly bear (Ursus arctos), polar bear (Ursus maritimus) and red fox (Vulpes vulpes). The most apt comparison is with walrus (Odobenus rosmarus), the only other pinniped in the DNA Zoo. The most recent common ancestor of the walrus and the seal lived ~35 million years ago. They show much stronger conservation of synteny. The data contrast helps illustrate the fact that the dog has one of the most extensively rearranged mammalian karyotypes investigated so far.


The genome assemblies for the walrus and dog used in the analysis can be found here and here, respectively.

Whole-genome alignment plots between the walrus (left) and domestic dog (right) to the Northern elephant seal genome assemblies.

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Writer's pictureRishi Ramesh

Updated: Apr 4, 2019

Brassica rapa is a plant that has many widely cultivated subspecies (cultivars). To name a few, the list includes turnip, napa cabbage, bomdong, bok choy and field mustard. Napa cabbage is a main ingredient in kimchi!


Today, we are sharing an update to the Brassica rapa reference Brapa_1.0 (GCA_000309985.1) from (Brassica rapa Genome Sequencing Project Consortium, Nat. Genet., 2011). See how the new reference aligns to Brassica oleracea, another commonly cultivated plant from the Brassica family, below. The genome assembly for B. oleracea used for whole genome alignment is Brassica_oleracea_HDEM.

Whole-genome alignment plot between B. rapa and B. oleracea genome assemblies.

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This week, we’re continuing our investigation of crocodilian genomics by assembling the chromosomes of the American alligator Alligator mississippiensis, the largest reptile in North America.


The new genome assembly is based on the draft data published in (St John et al., Genome Biology, 2012) and improved in (Rice et al., Genome Research, 2017). The sample used for Hi-C library preparation was donated by an alligator named Bo living at the Texas State Aquarium. That’s him on the blog post cover image (© Texas State Aquarium)!


Comparative gatoromics reveals nearly identical karyotypes. A different highly quantitative comparison is due to Sterling Archer.


Whole genome alignment of the 16 chromosomes of the new American alligator genome assembly ASM28112v4_HiC to the Chinese alligator chromosomes from ASM45574v1_HiC (see DNA Zoo blog post https://www.dnazoo.org/blog/alligator-bytes).

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