Chinese alligator (Alligator sinensis)

The Chinese alligator is widely regarded as the most endangered crocodilian in the world: fewer than 120 alligators left in the wild! Read more about Chinese alligators on WCS China website.

Chinese alligator, by Johan Wieland [CC BY-ND 2.0], from flickr.com

Chromosome-length genome assembly

Download the ASM45574v1.fasta.gz file containing the chromosome-length (2n=32) assembly of the Chinese alligator genome. All modifications with respect to the draft (see below) are annotated in the ASM45574v1.assembly file. Some basic stats associated with the new reference, ASM45574v1, are listed below. The full data release can be explored here.

Draft

The chromosome-length genome assembly is based on the draft assembly ASM45574v1 (GCF_000455745.1), credited below.

Wan, Qiu-Hong, Sheng-Kai Pan, Li Hu, Ying Zhu, Peng-Wei Xu, Jin-Quan Xia, Hui Chen, et al. 2013. "Genome Analysis and Signature Discovery for Diving and Sensory Properties of the Endangered Chinese Alligator." Cell Research 23 (9): 1091-1105. https://doi.org/10.1038/cr.2013.104.

Method

3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools  (Dudchenko et al., bioRxiv, 2018). See Methods for more information.

Hi-C sample

The blood sample for in situ Hi-C preparation was donated by a female individual named Princess Peach, and provided to us by Moody Gardens.

Hi-C Contact maps

Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). (Please note that the interactive figures are scaled 1:2.) To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools  (Dudchenko et al., bioRxiv, 2018).

References

If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.

Wan, Qiu-Hong, Sheng-Kai Pan, Li Hu, Ying Zhu, Peng-Wei Xu, Jin-Quan Xia, Hui Chen, et al. 2013. "Genome Analysis and Signature Discovery for Diving and Sensory Properties of the Endangered Chinese Alligator." Cell Research 23 (9): 1091-1105. https://doi.org/10.1038/cr.2013.104.

Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.

Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.

Disclaimer

This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at theaidenlab@gmail.com or leave a comment on the Forum.

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© 2018-2019 by the Aiden Lab.