Black petaltail dragonfly (Tanypteryx hageni)
The petaltail name recognizes the large, wide claspers at the end of the male abdomen (resembling the petals of a flower), which are used to clasp the female while mating. Read more about black petaltail dragonflies Christopher Beatty's website.
Adult Tanypteryx hageni, courtesy of Carey Kerst (used with permission)
Chromosome-length genome assembly
Download the tanypteryx.filtered_HiC.fasta.gz file containing the chromosome-length (n=9) assembly of the black petaltail dragonfly genome. All modifications with respect to the draft (see below) are annotated in the tanypteryx.filtered_HiC.assembly file. Some basic stats associated with the new reference, tanypteryx.filtered_HiC, are listed below. The full data release can be explored here.
Contig length (bp) | Number of contigs | Contig N50 (bp) | Longest contig (bp) |
|---|---|---|---|
1,694,818,643 | 2,133 | 4,275,485 | 23,896,077 |
Scaffold length (bp) | Number of scaffolds | Scaffold N50 (bp) | Longest scaffold (bp) |
|---|---|---|---|
1,695,269,643 | 1,231 | 206,643,428 | 277,860,391 |
Draft
The chromosome-length genome assembly is based on the draft assembly tanypteryx.filtered, credited below.
The draft genome assembly was generated by Ethan Tolman, Paul Frandsen, Jessica Ware, and Christopher Beatty at the American Museum of Natural History. Funding for this project was provided by the College Undergraduate Research Award from the College of Life Sciences at Brigham Young University. We also credit Grant #8709-09 from the National Geographic Society Committee for Research and Exploration.
Method
3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018). See Methods for more information.
Hi-C sample
The hemolymph sample for in situ Hi-C preparation was obtained from Christopher Beatty (American Museum of Natural History).
Hi-C Contact maps
Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools (Dudchenko et al., bioRxiv, 2018).
References
If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.
The draft genome assembly was generated by Ethan Tolman, Paul Frandsen, Jessica Ware, and Christopher Beatty at the American Museum of Natural History. Funding for this project was provided by the College Undergraduate Research Award from the College of Life Sciences at Brigham Young University. We also credit Grant #8709-09 from the National Geographic Society Committee for Research and Exploration.
Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.
Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.
Disclaimer
This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at thednazoo@gmail.com or leave a comment on the Forum.