Yellow fever mosquito (Aedes aegypti)

Aedes aegypti, the yellow fever mosquito, is a mosquito that can spread dengue fever, chikungunya, Zika fever, Mayaro and yellow fever viruses, and other disease agents. This mosquito originated in Africa, but is now found in tropical, subtropical and temperate regions throughout the world. Read more about yellow fever mosquitoes on WHO website.

Aedes aegypti, photo by James Gathany, Scientific Photography, Centers for Disease Control and Prevention

Chromosome-length genome assembly

Download the AaegL4.fasta.gz file containing the chromosome-length (2n=6) assembly of the yellow fever mosquito genome. All modifications with respect to the draft (see below) are annotated in the AaegL4.assembly file. Some basic stats associated with the new reference, AaegL4, are listed below. The full data release can be explored here. Note that chromosome-length genome assembly based on another draft is available for this species, here.

Draft

The chromosome-length genome assembly is based on the draft assembly AaegL3 (GCA_000004015.3), credited below.

Nene, V., Wortman, J.R., Lawson, D., Haas, B., Kodira, C., Tu, Z.J., Loftus, B., Xi, Z., Megy, K., Grabherr, M., Ren, Q., Zdobnov, E.M., Lobo, N.F., Campbell, K.S., Brown, S.E., Bonaldo, M.F., Zhu, J., Sinkins, S.P., Hogenkamp, D.G., Amedeo, P., Arensburger, P., Atkinson, P.W., Bidwell, S., Biedler, J., Birney, E., Bruggner, R.V., Costas, J., Coy, M.R., Crabtree, J., Crawford, M., Debruyn, B., Decaprio, D., Eiglmeier, K., Eisenstadt, E., El-Dorry, H., Gelbart, W.M., Gomes, S.L., Hammond, M., Hannick, L.I., Hogan, J.R., Holmes, M.H., Jaffe, D., Johnston, J.S., Kennedy, R.C., Koo, H., Kravitz, S., Kriventseva, E.V., Kulp, D., Labutti, K., Lee, E., Li, S., Lovin, D.D., Mao, C., Mauceli, E., Menck, C.F.M., Miller, J.R., Montgomery, P., Mori, A., Nascimento, A.L., Naveira, H.F., Nusbaum, C., O'leary, S., Orvis, J., Pertea, M., Quesneville, H., Reidenbach, K.R., Rogers, Y.-H., Roth, C.W., Schneider, J.R., Schatz, M., Shumway, M., Stanke, M., Stinson, E.O., Tubio, J.M.C., Vanzee, J.P., Verjovski-Almeida, S., Werner, D., White, O., Wyder, S., Zeng, Q., Zhao, Q., Zhao, Y., Hill, C.A., Raikhel, A.S., Soares, M.B., Knudson, D.L., Lee, N.H., Galagan, J., Salzberg, S.L., Paulsen, I.T., Dimopoulos, G., Collins, F.H., Birren, B., Fraser-Liggett, C.M., Severson, D.W., 2007. Genome sequence of Aedes aegypti, a major arbovirus vector. Science 316, 1718-1723. https://doi.org/10.1126/science.1138878.

Method

3D Assembly was performed using 3D-DNA pipeline (Dudchenko et al., Science, 2017). The genome was reviewed using Juicebox Assembly Tools  (Dudchenko et al., bioRxiv, 2018). See Methods for more information.

Hi-C sample

The sample for in situ Hi-C preparation was donated by a female individual, and obtained from Benzon Research.

Hi-C Contact maps

Hi-C data was aligned to the draft reference using Juicer (Durand, Shamim et al., Cell Systems, 2016), and contact maps visualizing the alignments with respect to the draft and the new reference were built using 3D-DNA (Dudchenko et al., Science, 2017). The contact maps can be explored below via Juicebox.js interactive tool (Robinson et al., Cell Systems, 2018). To explore the assembly in greater detail, please download the .hic and .assembly files from the data release folder and use Juicebox Assembly Tools  (Dudchenko et al., bioRxiv, 2018).

References

If you use this genome assembly in your research, please check that the conditions of use associated with the draft permit it, and acknowledge the following work.

Nene, V., Wortman, J.R., Lawson, D., Haas, B., Kodira, C., Tu, Z.J., Loftus, B., Xi, Z., Megy, K., Grabherr, M., Ren, Q., Zdobnov, E.M., Lobo, N.F., Campbell, K.S., Brown, S.E., Bonaldo, M.F., Zhu, J., Sinkins, S.P., Hogenkamp, D.G., Amedeo, P., Arensburger, P., Atkinson, P.W., Bidwell, S., Biedler, J., Birney, E., Bruggner, R.V., Costas, J., Coy, M.R., Crabtree, J., Crawford, M., Debruyn, B., Decaprio, D., Eiglmeier, K., Eisenstadt, E., El-Dorry, H., Gelbart, W.M., Gomes, S.L., Hammond, M., Hannick, L.I., Hogan, J.R., Holmes, M.H., Jaffe, D., Johnston, J.S., Kennedy, R.C., Koo, H., Kravitz, S., Kriventseva, E.V., Kulp, D., Labutti, K., Lee, E., Li, S., Lovin, D.D., Mao, C., Mauceli, E., Menck, C.F.M., Miller, J.R., Montgomery, P., Mori, A., Nascimento, A.L., Naveira, H.F., Nusbaum, C., O'leary, S., Orvis, J., Pertea, M., Quesneville, H., Reidenbach, K.R., Rogers, Y.-H., Roth, C.W., Schneider, J.R., Schatz, M., Shumway, M., Stanke, M., Stinson, E.O., Tubio, J.M.C., Vanzee, J.P., Verjovski-Almeida, S., Werner, D., White, O., Wyder, S., Zeng, Q., Zhao, Q., Zhao, Y., Hill, C.A., Raikhel, A.S., Soares, M.B., Knudson, D.L., Lee, N.H., Galagan, J., Salzberg, S.L., Paulsen, I.T., Dimopoulos, G., Collins, F.H., Birren, B., Fraser-Liggett, C.M., Severson, D.W., 2007. Genome sequence of Aedes aegypti, a major arbovirus vector. Science 316, 1718-1723. https://doi.org/10.1126/science.1138878.

Dudchenko, O., Batra, S.S., Omer, A.D., Nyquist, S.K., Hoeger, M., Durand, N.C., Shamim, M.S., Machol, I., Lander, E.S., Aiden, A.P., Aiden, E.L., 2017. De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds. Science 356, 92–95. https://doi.org/10.1126/science.aal3327.

Dudchenko, O., Shamim, M.S., Batra, S., Durand, N.C., Musial, N.T., Mostofa, R., Pham, M., Hilaire, B.G.S., Yao, W., Stamenova, E., Hoeger, M., Nyquist, S.K., Korchina, V., Pletch, K., Flanagan, J.P., Tomaszewicz, A., McAloose, D., Estrada, C.P., Novak, B.J., Omer, A.D., Aiden, E.L., 2018. The Juicebox Assembly Tools module facilitates de novo assembly of mammalian genomes with chromosome-length scaffolds for under $1000. bioRxiv 254797. https://doi.org/10.1101/254797.

Disclaimer

This is a work in progress. If you notice any discrepancies in the map or have data that confirms or contradicts the suggested reference, please email us at theaidenlab@gmail.com or leave a comment on the Forum.

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© 2018-2020 by the Aiden Lab.